Gene expression in sheep cumulus-oocyte complexes meiotically inhibited with roscovitine
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چکیده
منابع مشابه
In vitro effects of relaxin on gene expression in porcine cumulus-oocyte complexes and developing embryos
BACKGROUND Relaxin hormone peptide is found in porcine follicular and utero-tubal fluids, but its possible actions during early embryo development are still undetermined. Here, we investigated the effects of porcine relaxin during oocyte maturation and embryo development, and gene expression in the pig. METHODS Immature cumulus-oocyte complexes (COCs) were obtained from ovarian follicles of s...
متن کاملGene Expression in Cumulus Cells and Oocyte Quality
A.R. Genazzani, B.C. Tarlatzis (eds.), Frontiers in Gynecological Endocrinology: Volume 3: Ovarian Function and Reproduction From Needs to Possibilities, ISGE Series, DOI 10.1007/978-3-319-23865-4_5 P.G. Artini Division of Gynecology and Obstetrics, Department of Experimental and Clinical Medicine, University of Pisa, Pisa, Italy e-mail: [email protected] 5 Gene Expression in Cumulus Ce...
متن کاملP-110: Effect of Increasing Amount of Oocyte Secreted Factors on Cumulus Expansion of Bovine Cumulus-Oocyte Complexes
Background: In vitro maturation is a good method to decrease cancer risk of superovulation by gonadotropin hormones. A paracrine effect of oocyte secretions on oocyte developmental competence is under investigation. Apart from oocyte maturation, ovulation in vivo requires a precise control of extracellular matrix modification. Cumulus cells secrete hyaluronan to form a muco-elastic extracellula...
متن کاملThe human cumulus--oocyte complex gene-expression profile.
BACKGROUND The understanding of the mechanisms regulating human oocyte maturation is still rudimentary. We have identified transcripts differentially expressed between immature and mature oocytes and cumulus cells. METHODS Using oligonucleotide microarrays, genome-wide gene expression was studied in pooled immature and mature oocytes or cumulus cells from patients who underwent IVF. RESULTS...
متن کاملCryobiological effects of cryoprotectants on morphology of cumulus oocyte complexes (COCs) of sheep using vitrification
The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocyte using vitrification. The vitrification of sheep oocyte was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more e...
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ژورنال
عنوان ژورنال: Small Ruminant Research
سال: 2015
ISSN: 0921-4488
DOI: 10.1016/j.smallrumres.2015.10.014